Rivista
/
Neuroscienze
/
Magnetic Isolation of Microglial Cells from Neonate Mouse for Primary Cell Cultures
JoVE Journal
Neuroscienze
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Neuroscienze
Magnetic Isolation of Microglial Cells from Neonate Mouse for Primary Cell Cultures

Magnetic Isolation of Microglial Cells from Neonate Mouse for Primary Cell Cultures

DOI:
10.3791/62964-v

•

07:23 min

•

July 25, 2022

•

, , , , , , , , , ,
1NeuroDiderot, Inserm UMR-1141, Hôpital Robert Debré 48,Université de Paris, 2Department of anesthesia and critical care,APHP-Sorbonne university

Capitoli

  • 00:05Introduction
  • 00:32Brain Dissection
  • 01:29Brain Dissociation and Magnetic Microglial Isolation
  • 04:23Phagocytic Assay
  • 05:03Results: Phagocytic Activity and Magnetic Isolation of Microglia In Vitro
  • 06:49Conclusion

Summary

Traduzione automatica

Traduzione automatica

Primary microglia cultures are commonly used to evaluate new anti-inflammatory molecules. The present protocol describes a reproducible and relevant method to magnetically isolate microglia from neonate pups.

Tags

Keywords: MicrogliaMagnetic Cell SortingNeonate MousePrimary Cell CultureCD11bCell DissociationHBSSSorting BufferCell SeparationCell Count

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/62964?article_type=v

Multiple-mouse Neuroanatomical Magnetic Resonance Imaging
check_url/it/62964?article_type=v

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
check_url/it/62964?article_type=v

One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice
check_url/it/62964?article_type=v

Isolation of Primary Murine Brain Microvascular Endothelial Cells
check_url/it/62964?article_type=v

Preparation of Primary Mixed Glial Cultures from Adult Mouse Spinal Cord Tissue
check_url/it/62964?article_type=v

Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility
check_url/it/62964?article_type=v

Isolation and Culture of Embryonic Mouse Neural Stem Cells
check_url/it/62964?article_type=v

Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells
check_url/it/62964?article_type=v

Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum
check_url/it/62964?article_type=v

Isolation of Mouse Primary Microglia by Magnetic-Activated Cell Sorting in Animal Models of Demyelination

Read Article