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两种剥离方法，用于分离小鼠视网膜中的光感受器细胞区室以进行蛋白质分析
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Neuroscienze
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JoVE Journal Neuroscienze
Two Peeling Methods for the Isolation of Photoreceptor Cell Compartments in the Mouse Retina for Protein Analysis
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两种剥离方法，用于分离小鼠视网膜中的光感受器细胞区室以进行蛋白质分析

DOI:
10.3791/62977-v

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11:08 min

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December 07, 2021

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, ,
1Zilkha Neurogenetic Institute, Keck School of Medicine,University of Southern California, 2Department of Cell & Neurobiology, Keck School of Medicine,University of Southern California

Capitoli

  • 00:04Introduction
  • 00:52Live Cell Retinal Peeling Method
  • 03:35Lyophilized Retina Peeling Method
  • 07:42Results: Isolation of Subcellular Compartments of Murine Rod Photoreceptors for Protein Analysis
  • 10:23Conclusion

Summary

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该协议提出了两种技术来分离小鼠杆光感受器的亚细胞区室以进行蛋白质分析。第一种方法利用活视网膜和纤维素滤纸来分离杆外段，而第二种方法采用冻干视网膜和胶带剥离杆内段和外段层。

Tags

Photoreceptor Cell CompartmentsMouse RetinaProtein AnalysisPeeling TechniquesRod Subcellular CompartmentsRod Outer Segment (ROS)Ames HEPES BufferFilter PaperC57 Black 6J MouseRetinal DissectionProtein Analysis

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