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使用Y-27632的原发性人类金吉瓦尔表皮细胞的隔离与培养
JoVE Journal
Bioingegneria
Author Produced
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JoVE Journal Bioingegneria
Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632
DOI:

06:47 min

November 06, 2021

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Capitoli

  • 00:00Introduction
  • 00:49Gingival Tissue Pretreatment
  • 01:13Digestion of Gingival Tissue
  • 02:46Cell Passage
  • 03:51Representative Results
  • 06:18Conclusion

Summary

Traduzione automatica

在这里,我们提出了一个改进的方法,通过添加岩石抑制剂,Y-27632,以传统的方法隔离和培养人类金吉瓦尔上皮细胞。这种方法更容易,更省时,提高干细胞的特性,并产生更多的高潜力上皮细胞,无论是为实验室和临床应用。

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