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An Explant System for Time-Lapse Imaging Studies of Olfactory Circuit Assembly in Drosophila
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JoVE Journal Biologia dello sviluppo
An Explant System for Time-Lapse Imaging Studies of Olfactory Circuit Assembly in Drosophila

An Explant System for Time-Lapse Imaging Studies of Olfactory Circuit Assembly in Drosophila

DOI:
10.3791/62983-v

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07:06 min

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October 13, 2021

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1Department of Biology, Howard Hughes Medical Institute, Department of Biology,Stanford University

Capitoli

  • 00:05Introduction
  • 00:44Reagent Preparation
  • 02:44Explant Dissection
  • 05:31Results: Genetic Identification of the Extracted ORN Axons
  • 06:10Conclusion

Summary

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This protocol describes the dissection procedure, culture condition, and live imaging of an antennae-brain explant system for the study of the olfactory circuit assembly.

Tags

Keywords: Olfactory CircuitTime-lapse ImagingDrosophilaExplant SystemBrain DissectionMicrodissectionPupaeCulture MediumSylgard PlateMicro PinsHeat ShockSchneider’s Drosophila MediumFetal Bovine SerumHuman Insulin20-hydroxyecdysone

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