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实时监测细胞因子分化人原代T细胞中的线粒体呼吸

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells

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实时监测细胞因子分化人原代T细胞中的线粒体呼吸

DOI:

10.3791/62984-v

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06:55 min

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October 19, 2021

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Kasper Mølgaard*¹, Anne Rahbech*¹, Özcan Met, Inge Marie Svane, Per thor Straten³, Claus Desler, Marlies J. W. Peeters

¹National Center for Cancer Immune Therapy, Department of Oncology,University Hospital Herlev, ²Department of Cellular and Molecular Medicine, Center for Healthy Aging,University of Copenhagen, ³Department of Immunology and Microbiology, Inflammation and Cancer Group,University of Copenhagen

Capitoli

00:05Introduction
00:39Activating Human Primary T Lymphocytes
01:55Extracellular Flux Assay
04:52Results: Optimizing Mitochondrial Modulator Concentrations and Cell Numbers for Monitoring Mitochondrial Respiration in Human Primary T Cells
06:26Conclusion

Summary

Traduzione automatica

代谢适应是 T 细胞的基础，因为它决定了分化、持久性和细胞毒性。本文介绍了一种用于监测离体细胞因子分化的人原代T细胞中线粒体呼吸的优化方案。

Tags

Keywords: Mitochondrial Respiration T Cell Cytokine Seahorse Machine Extracellular Flux Assay Cell-Tak XF Cell Culture Plate Assay Media Glucose Pyruvate Glutamine

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