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冈 比亚按蚊 幼虫唾液腺的解剖和免疫染色
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Biologia
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JoVE Journal Biologia
Dissection and Immunostaining of Larval Salivary Glands from Anopheles gambiae Mosquitoes
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冈 比亚按蚊 幼虫唾液腺的解剖和免疫染色

DOI:
10.3791/62989-v

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06:12 min

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September 30, 2021

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, , , ,
1Department of Cell Biology,The Johns Hopkins University School of Medicine, 2Tufts School of Medicine, 3Department of Biomedical Sciences,Idaho College of Osteopathic Medicine

Capitoli

  • 00:04Introduction
  • 00:46Salivary Gland Dissection
  • 02:01Fixation and Immunostaining
  • 03:25Mounting Stained Glands for Microscopy
  • 04:25Results: Morphology of L4 Stage Mosquito and Salivary Gland Dissected from Mosquito Head
  • 05:41Conclusion

Summary

Traduzione automatica

Traduzione automatica

成年蚊子唾液腺（SG）是将所有蚊子传播的病原体（包括病毒和寄生虫）传播给人类宿主所必需的。该视频演示了从幼虫（L4）阶段冈 比亚按蚊 中有效分离SG以及L4 SGs的制备以进行进一步分析。

Tags

Salivary GlandsAnopheles GambiaeMosquito LarvaeDissectionImmunostainingDisease TransmissionMalarial ParasitesRab 11Alexa Fluor 488Nile RedHoechst

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