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High-Resolution Respirometry to Assess Bioenergetics in Cells and Tissues Using Chamber- and Plate-Based Respirometers
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Biologia
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JoVE Journal Biologia
High-Resolution Respirometry to Assess Bioenergetics in Cells and Tissues Using Chamber- and Plate-Based Respirometers

High-Resolution Respirometry to Assess Bioenergetics in Cells and Tissues Using Chamber- and Plate-Based Respirometers

DOI:
10.3791/63000-v

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09:53 min

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October 26, 2021

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,
1Department of Biochemistry and Developmental Biology, Faculty of Medicine,University of Helsinki

Capitoli

  • 00:04Introduction
  • 01:01High-Resolution Respirometry: Chamber-Based Respirometer (cHRR)
  • 04:51High-Resolution Respirometry: Microplate-Based Respirometer (mHRR)
  • 06:48Results: Oxygen Consumption Traces from cHRR and mHRR Using HEK293 Cells with Combined OXPHOS Deficiency
  • 08:53Conclusion

Summary

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Assessing oxidative phosphorylation using high-resolution respirometers has become an integral part of the functional analysis of mitochondria and cellular energy metabolism. Here, we present protocols for the analysis of cellular energy metabolism using chamber and microplate-based high-resolution respirometers and discuss the key benefits of each device.

Tags

Keywords: High-resolution RespirometryBioenergeticsMitochondrial DysfunctionOxidative PhosphorylationHEK293 CellsOXPHOS AnalysisOligomycinFCCPRotenoneAntimycin

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