Rivista
/
Genetica
/
Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti
JoVE Journal
Genetica
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Genetica
Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti

Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti

DOI:
10.3791/63008-v

•

05:30 min

•

September 10, 2021

•

, , , ,
1Department of Entomology and Agrilife Research,Texas A&M University

Capitoli

  • 00:05Introduction
  • 00:33A Gradient PCR Using Genomic DNA (gDNA) from a Single Individual
  • 01:09Preparation of Genomic DNA from Mosquito Legs
  • 02:31Perform PCR
  • 03:35Results: Detection of CRISPR/Cas9-Induced Indels and the Establishment of Mutant Lines in the Mosquito Aedes aegypti
  • 05:02Conclusion

Summary

Traduzione automatica

Traduzione automatica

This article details a protocol for rapid identification of indels induced by CRISPR/Cas9 and selection of mutant lines in the mosquito Aedes aegypti using high-resolution melt analysis.

Tags

CRISPR/Cas9Indel DetectionHigh-resolution Melt AnalysisAedes AegyptiMosquitoPrimer DesignGradient PCRDNA ReleaseThermal Melt ProfilePCR Plate

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus

High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

Introducing a Gene Knockout Directly Into the Amastigote Stage of Trypanosoma cruzi Using the CRISPR/Cas9 System

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

Read Article