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Prøveforberedelse til computertomografibaseret tredimensionel visualisering af Murine Hind-limb Vessels

JoVE Journal
Medicina

This content is Free Access.

JoVE Journal Medicina

Sample Preparation for Computed Tomography-based Three-dimensional Visualization of Murine Hind-limb Vessels

Prøveforberedelse til computertomografibaseret tredimensionel visualisering af Murine Hind-limb Vessels

DOI:

10.3791/63009-v

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04:35 min

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October 07, 2021

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Daiki Seya, Yuqing Xu³, Toshifumi Mukunoki, Kenichi Tsujita, Osamu Nakagawa, Yuichiro Arima^2,3,4

¹Department of Molecular Physiology,National Cerebral and Cardiovascular Center Research Institute, ²International Research Center for Medical Sciences (IRCMS),Kumamoto University, ³Department of Cardiovascular Medicine, Graduate School of Medical Sciences,Kumamoto University, ⁴X-Earth Center, Faculty of Advanced Science and Technology,Kumamoto University

Capitoli

00:04Introduction
00:46Perfusion
02:56Results: 3D Evaluation of Murine Blood Vessels in The Lower Extremities of the Mouse
03:58Conclusion

Summary

Traduzione automatica

Her beskriver vi en visualiserings- og kvantificeringsmetode for murine bagbenskar ved hjælp af mikro-røntgen computertomografi.

Tags

Keywords: Computed Tomography 3D Visualization Murine Hind-limb Vessels Sample Preparation Heparin Vasodilation Buffer Paraformaldehyde Contrast Medium Perfusion Hind-limb Ischemia Model Femoral Artery Femoral Vein Collateral Vessels

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