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Bioengineering

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JoVE Journal Bioengineering
En high-yield Streptomyces Transskription-Oversættelse Toolkit til syntetisk biologi og naturlige produktapplikationer
 
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En high-yield Streptomyces Transskription-Oversættelse Toolkit til syntetisk biologi og naturlige produktapplikationer

Article DOI: 10.3791/63012-v • 07:59 min • September 10th, 2021
September 10th, 2021 •
1,2,3,4, 5, 5, 1,2,3,4,6,7, 5
1Centre for Synthetic Biology and Innovation, South Kensington Campus, 2Department of Medicine, South Kensington Campus, 3Section of Structural and Synthetic Biology, Department of Infectious Disease, Imperial College London, 4Sir Alexander Fleming Building, South Kensington Campus, 5School of Biosciences, Division of Natural Sciences, University of Kent, 6UK Dementia Research Institute Care Research and Technology Centre, Imperial College London; Hammersmith Campus, 7UK Innovation and Knowledge Centre for Synthetic Biology (SynbiCITE) and the London Biofoundry, Imperial College Translation & Innovation Hub

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Denne protokol beskriver en forbedret metode til syntetisering af høje udbytter af rekombinante proteiner fra en Streptomyces venezuelae cellefri transskription-oversættelse (TX-TL) system.

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Bioengineering Udgave 175 Cellefri proteinsyntese in vitro transskription-oversættelse Streptomyces syntetisk biologi systembiologi cellefri systemer biosyntese
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