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Detecting Virus and Salivary Proteins of a Leafhopper Vector in the Plant Host
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Biologia
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JoVE Journal Biologia
Detecting Virus and Salivary Proteins of a Leafhopper Vector in the Plant Host

Detecting Virus and Salivary Proteins of a Leafhopper Vector in the Plant Host

DOI:
10.3791/63020-v

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07:23 min

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September 14, 2021

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, , ,
1Vector-borne Virus Research Center, Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology,Fujian Agriculture and Forestry University

Capitoli

  • 00:05Introduction
  • 00:35Non-Viruliferous Insect Rearing
  • 01:29Virus Acquisition and Collection of Viruliferous Insects
  • 02:10Collection of Salivary Proteins Using a Feeding Cage
  • 03:04Reagent Preparation
  • 03:51Detection of Saliva and Viral Proteins by Western Blotting
  • 06:02Results: Western Blot Analysis for Detection of Viral and Salivary Proteins of a Leafhopper Vector in the Plant Host
  • 06:51Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol demonstrates how to use the plant host to detect salivary proteins of leafhopper and plant viral proteins released by leafhopper vectors.

Tags

LeafhopperSalivary ProteinsPlant HostVirus DetectionVirus TransmissionRice Dwarf VirusFeeding CageAspiratorNon-viruliferous NymphsCirculative TransmissionInsect-proof NetTris-Glycine Buffer

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