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检测植物宿主中叶蝉载体的病毒和唾液蛋白
JoVE Journal
Biologia
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JoVE Journal Biologia
Detecting Virus and Salivary Proteins of a Leafhopper Vector in the Plant Host
DOI:

07:23 min

September 14, 2021

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Capitoli

  • 00:05Introduction
  • 00:35Non-Viruliferous Insect Rearing
  • 01:29Virus Acquisition and Collection of Viruliferous Insects
  • 02:10Collection of Salivary Proteins Using a Feeding Cage
  • 03:04Reagent Preparation
  • 03:51Detection of Saliva and Viral Proteins by Western Blotting
  • 06:02Results: Western Blot Analysis for Detection of Viral and Salivary Proteins of a Leafhopper Vector in the Plant Host
  • 06:51Conclusion

Summary

Traduzione automatica

该协议演示了如何使用植物宿主来检测叶蝉的唾液蛋白和叶蝉载体释放的植物病毒蛋白。

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