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Het detecteren van virus- en speekseleiwitten van een leafhoppervector in de plantengastheer
JoVE Journal
Biologia
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JoVE Journal Biologia
Detecting Virus and Salivary Proteins of a Leafhopper Vector in the Plant Host
DOI:

07:23 min

September 14, 2021

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Capitoli

  • 00:05Introduction
  • 00:35Non-Viruliferous Insect Rearing
  • 01:29Virus Acquisition and Collection of Viruliferous Insects
  • 02:10Collection of Salivary Proteins Using a Feeding Cage
  • 03:04Reagent Preparation
  • 03:51Detection of Saliva and Viral Proteins by Western Blotting
  • 06:02Results: Western Blot Analysis for Detection of Viral and Salivary Proteins of a Leafhopper Vector in the Plant Host
  • 06:51Conclusion

Summary

Traduzione automatica

Dit protocol laat zien hoe de plantengastheer kan worden gebruikt om speekseleiwitten van leafhopper en plantaardige virale eiwitten te detecteren die worden vrijgegeven door leafhopper-vectoren.

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