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1Department of Pathology,University of Michigan, Ann Arbor, 2Department of Internal Medicine,University of Michigan, Ann Arbor, 3Department of Cellular and Developmental Biology,University of Michigan, Ann Arbor, 4Rogel Cancer Center,University of Michigan, Ann Arbor, 5Institute of Gerontology,University of Michigan, Ann Arbor
Capitoli
- 00:05Introduction
- 01:08Hydration of the Sensor Cartridge
- 02:03Preparation of Cell-Adhesive-Coated Microplates
- 02:52Seeding Cells in Cell-Adhesive-Coated Microplates
- 04:10Glycolysis Stress Test Using the Extracellular Flux Analyzer
- 07:53Results: Assessment of the Glycolytic Function and Mitochondrial Respiration in Mouse HSPCs
- 09:36Conclusion
O método aqui apresentado resume protocolos otimizados para avaliação de bioenergésicos celulares em haste hematopoiéticas não aderentes e células progenitoras primitivas (HSPCs) utilizando o analisador de fluxo extracelular para medir a taxa de acidificação extracelular (ECAR) e a taxa de consumo de oxigênio (OCR) dos HSPCs em tempo real.
Tags
Keywords:
Hematopoietic Stem CellsHematopoiesisGlycolysisOxidative PhosphorylationCellular BioenergeticsExtracellular Flux AnalyzerMetabolic SwitchMouse Hematopoietic Stem And Progenitor CellsHigh Throughput QuantificationMetabolic PathwaysPluripotencySuspension CellsCell Adhesive Solution96-well Microplate
