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用于研究真核糖原代谢的分光光度法

JoVE Journal
Biochimica

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JoVE Journal Biochimica

Spectrophotometric Methods for the Study of Eukaryotic Glycogen Metabolism

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用于研究真核糖原代谢的分光光度法

DOI:

10.3791/63046-v

•

07:59 min

•

August 19, 2021

•

Wayne A. Wilson

¹Department of Biochemistry & Nutrition,Des Moines University

Capitoli

00:05Introduction
00:27Determination of Glycogen Synthase Activity
02:28Measurement of Released Glucose
03:55Determination of Glycogen Branching
05:34Results: Spectrophotometric Assays of Glycogen Synthase
07:31Conclusion

Summary

Traduzione automatica

介绍了使用在可见光范围内操作的简单分光光度计测量糖原代谢关键酶活性的技术。

Tags

Keywords: Glycogen Metabolism Spectrophotometric Methods Glycogen Synthase Activity UDP Glucose NADH Glycogen Glucose-6-phosphate Dehydrogenase Hexokinase Eukaryotic Metabolism

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