/
/
Improving Reproducibility to Meet Minimal Information for Studies of Extracellular Vesicles 2018 Guidelines in Nanoparticle Tracking Analysis
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:04Introduction
- 00:44Cleaning and Assembly of the Laser Module
- 01:29Flushing Procedure for the Laser Module Prior to and Between Samples
- 02:28Focusing and Positioning of the Laser Module
- 03:34Loading Standards/Samples/Diluent into the Laser Module for Nanoparticle Tracking Analysis (NTA)
- 04:28Sample Nanoparticle Tracking Analysis (NTA)
- 06:06Re-Analysis of the Current Sample at Different Detection Threshold
- 06:40Results: Effects of Camera Level and Detection Threshold on Measured Particle Size and Concentration of Filtered and Unfiltered Samples
- 08:02Conclusion
Nanoparticle tracking analysis (NTA) is a widely used method to characterize extracellular vesicles. This paper highlights NTA experimental parameters and controls plus a uniform method of analysis and characterization of samples and diluents necessary to supplement the guidelines proposed by MISEV2018 and EV-TRACK for reproducibility between laboratories.
