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Direct and Indirect Culture Methods for Studying Biodegradable Implant Materials In Vitro
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Capitoli
- 00:07Introduction
- 02:04Cell Culture Preparation
- 02:20Harvesting Bone Marrow Derived Mesenchymal Stem Cells (BMSCs) from Rat Weanlings
- 04:04Sample Preparation and Sterilization
- 04:25Cell Culture Methods
- 06:52Postculture Characterization of Cells
- 10:22Postculture Analyses of Media and Samples
- 11:55Representative Results
- 14:06Conclusions
We introduce three methods of direct culture, direct exposure culture, and exposure culture for evaluating the in vitro cytocompatibility of biodegradable implant materials. These in vitro methods mimic different in vivo cell-implant interactions and can be applied to study various biodegradable materials.
