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使用比率指标对原代神经元中的线粒体谷胱甘肽氧化还原状态进行实时成像

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator

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使用比率指标对原代神经元中的线粒体谷胱甘肽氧化还原状态进行实时成像

DOI:

10.3791/63073-v

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07:47 min

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October 20, 2021

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Athanasios Katsalifis, Angela Maria Casaril, Constanze Depp, Carlos Bas-Orth

¹Department of Medical Cell Biology, Institute for Anatomy and Cell Biology,Heidelberg University, ²Department of Neurogenetics,Max-Planck-Institute for Experimental Medicine

Capitoli

00:05Introduction
00:55Optimization of Scanning Confocal Microscope Settings
01:58Live Imaging of the Mitochondrial Redox Response to N-methyl-D-aspartate (NMDA) Treatment
03:08Data Analysis
05:40Results: Effects of N-methyl-D-aspartate (NMDA) on Neuronal Mitochondria Oxidation
07:04Conclusion

Summary

Traduzione automatica

本文介绍了一种方案，用于使用共聚焦活显微镜确定原代海马和皮质神经元中基底氧化还原状态和氧化还原反应的差异。该协议可以应用于其他细胞类型和显微镜，只需最少的修改。

Tags

Keywords: Mitochondrial Glutathione Redox State Live Imaging Primary Neurons Ratiometric Indicator Mitochondrial Redox State Mitochondrial Membrane Potential Calcium Concentrations Confocal Microscopy NMDA DTT

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