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多发性骨髓瘤骨髓涂片的相间荧光原位杂交

JoVE Journal
Medicina
Author Produced

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JoVE Journal Medicina

Interphase Fluorescence in situ Hybridization of Bone Marrow Smears of Multiple Myeloma

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多发性骨髓瘤骨髓涂片的相间荧光原位杂交

DOI:

10.3791/63083-v

•

07:53 min

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April 15, 2022

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Yalan Yu*¹, Hui Shen*¹, Li Liu, Ping Luo, Sanyun Wu, Jing He², Xiqin Tong, Yufeng Shang, Liang Shao, Fuling Zhou

¹Department of Hematology,Zhongnan Hospital of Wuhan University, ²Wuhan National Laboratory for Optoelectronics,Huazhong University of Science and Technology

Capitoli

00:09Introduction
01:23Preparation of Bone Marrow Smears
02:37FISH Pre-Processing of Bone Marrow Smear
03:48Bone Marrow Smear FISH and Washing
05:20FISH Analysis and Imaging
06:20Representative Result
07:22Conclusion

Summary

Traduzione automatica

在这里，我们提出了一种方案，用于提高相间荧光原位杂交检测对多发性骨髓瘤患者骨髓涂片的成功率。

Tags

Interphase Fluorescence In Situ Hybridization Bone Marrow Smears Multiple Myeloma Plasma Cells CIg FISH Genetic Risk Stratification Wright-Giemsa Staining Microscopy Fixative Solution SSC Buffer

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