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De op microscopie gebaseerde test om de recycling-endosomen te bestuderen en te analyseren met behulp van SNARE-handel
JoVE Journal
Biologia
Author Produced
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JoVE Journal Biologia
The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
DOI:

08:51 min

February 12, 2022

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Capitoli

  • 00:06Introduction
  • 01:04Seeding of Mouse Melanocytes on Pre-Treated Coverslips
  • 01:42Transfection of Cells with the STX13 Plasmids
  • 02:39Fixation of the Cells
  • 03:11Immunostaining of the Cells
  • 05:05Results: Quantification of Overlap Between the RE Localized Proteins and Melanosomes
  • 07:25Results: Quantification of Recycling Endosomes’ Tublar Number and Length
  • 07:56Conclusion

Summary

Traduzione automatica

Recycling endosomen maken deel uit van het endosomale buisvormige netwerk. Hier presenteren we een methode om de dynamiek van recycling-endosomen te kwantificeren met behulp van GFP-STX13 als organelmarker.

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