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尿嘧啶-DNA糖基化酶测定,采用基质辅助激光解吸/电离飞行时间质谱分析
JoVE Journal
Biochimica
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JoVE Journal Biochimica
Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis
DOI:

12:19 min

April 22, 2022

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Capitoli

  • 00:04Introduction
  • 01:28DNA Glycosylase Assay
  • 04:43Transferring UDG Reaction Products to a Matrix Chip
  • 05:33Setting up the Assay Parameters on the Mass Spectrometer
  • 07:19Mass Spectrometer Operation, Viewing Mass Spectra, and Analyzing the Data
  • 09:03Results: UDG Assay Using MALDI-TOF MS for AP Site Containing Product Analysis
  • 11:01Conclusion

Summary

Traduzione automatica

使用MALDI-TOF质谱法开发了一种非标记,非放射性同位素方法来测定尿嘧啶-DNA糖基化酶活性,用于直接分析含阳离子/嘧啶位点的产品。该测定被证明是非常简单,特异性,快速且易于使用的DNA糖基化酶测量。

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