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Uracil-DNA Glycosylase Assay door Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis
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1Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine,National Taiwan University, 2Department of Laboratory Medicine,National Taiwan University Hospital, 3Center for Microbial Pathogenesis, Nationwide Children’s Hospital and the Department of Pediatrics,The Ohio State University
Capitoli
- 00:04Introduction
- 01:28DNA Glycosylase Assay
- 04:43Transferring UDG Reaction Products to a Matrix Chip
- 05:33Setting up the Assay Parameters on the Mass Spectrometer
- 07:19Mass Spectrometer Operation, Viewing Mass Spectra, and Analyzing the Data
- 09:03Results: UDG Assay Using MALDI-TOF MS for AP Site Containing Product Analysis
- 11:01Conclusion
Een niet-gelabelde, niet-radio-isotopische methode om uracil-DNA glycosylase activiteit te testen werd ontwikkeld met behulp van MALDI-TOF massaspectrometrie voor directe apurine / apyrimidinische site-bevattende productanalyse. De test bleek vrij eenvoudig, specifiek, snel en gemakkelijk te gebruiken voor DNA-glycosylasemeting.
