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Sammlung, Expansion und Differenzierung primärer humaner Nasensepithelzellmodelle zur Quantifizierung der Zilienschlagfrequenz
 
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Sammlung, Expansion und Differenzierung primärer humaner Nasensepithelzellmodelle zur Quantifizierung der Zilienschlagfrequenz

Article DOI: 10.3791/63090-v • 11:13 min • November 10th, 2021
November 10th, 2021 •
1,2, 1,2, 1,2,3, 1,2, 1,2,3, 4, 5, 1,2,3
1School of Women's and Children's Health, Faculty of Medicine and Health, University of New South Wales, 2Molecular and Integrative Cystic Fibrosis Research Centre (miCF RC), Faculty of Medicine and Health, University of New South Wales, 3Department of Respiratory Medicine, Sydney Children's Hospital, 4Department of Pathology, School of Medical Sciences, University of New South Wales Australia, 5Katharina Gaus Light Microscopy Facility, Mark Wainwright Analytical Centre, University of New South Wales

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Dieses Protokoll beschreibt die Entnahme, Expansion und Differenzierung nasaler Epithelzellen zu organotypischen Atemwegsepithelzellmodellen und die Quantifizierung der Zilienschlagfrequenz mittels Live-Cell-Bildgebung und maßgeschneiderten Skripten.

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Medizin Ausgabe 177
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