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Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells
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Capitoli
- 00:04Introduction
- 00:59Generation of 3D Self-Assembling Human Heart Organoids
- 02:51Human Heart Organoid (hHO) Differentiation
- 05:33Organoid Analysis
- 06:03Results: Self-organizing hHO
- 08:02Conclusion
Here, we describe a protocol to create developmentally relevant human heart organoids (hHOs) efficiently using human pluripotent stem cells by self-organization. The protocol relies on the sequential activation of developmental cues and produces highly complex, functionally relevant human heart tissues.
Tags
Self-assemblingHuman HeartOrganoidsPluripotent Stem Cells3D StructureDeveloping HeartCardiovascular DiseasesPharmacological TreatmentsHigh ThroughputCongenital Heart DefectsEmbryoid BodiesCell CultureDifferentiationHPSCDPBSACUTAPSC MediumThiasCell SuspensionCell CounterHemo CytometerRound Bottom Ultra Low Attachment 96 Well PlateRPMI 1640Insulin Free B27 Supplement
