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Bioengineering

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JoVE Journal Bioengineering
A Micropatterning Assay for Measuring Cell Chirality
 

A Micropatterning Assay for Measuring Cell Chirality

Article DOI: 10.3791/63105-v • 08:07 min • March 11th, 2022
March 11th, 2022 •
1,2, 3, 3,4, 1,2,5,6
1Department of Biomedical Engineering, Rensselaer Polytechnic Institute, 2Center for Biotechnology & Interdisciplinary Studies, Rensselaer Polytechnic Institute, 3Department of Biomedical Engineering, Columbia University, 4Department of Medicine, Columbia University, 5Department of Biological Sciences, Rensselaer Polytechnic Institute, 6Center for Modeling, Simulation, and Imaging in Medicine, Rensselaer Polytechnic Institute

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We present a protocol for determining multicellular chirality in vitro, using the micropatterning technique. This assay allows for automatic quantification of the left-right biases of various types of cells and can be used for screening purposes.

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Micropatterning Assay Cell Chirality Multicellular Chiral Morphogenesis Disease Development Cell Research Macropattern System Titanium Gold-coated Slide Glass Cutter Ethanol Cleaning PDMS Stamps C18 Solution Stamping Technique
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