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Neuroscience

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JoVE Journal Neuroscience
Flow Cytometric Analysis of Multiple Mitochondrial Parameters in Human Induced Pluripotent Stem Cells and Their Neural and Glial Derivatives
 

Flow Cytometric Analysis of Multiple Mitochondrial Parameters in Human Induced Pluripotent Stem Cells and Their Neural and Glial Derivatives

Article DOI: 10.3791/63116-v • 06:09 min • November 8th, 2021
November 8th, 2021 •
*1,2, *1,2,3,4, 1,2, 1,2
1Department of Clinical Medicine (K1), University of Bergen, 2Neuro-SysMed, Center of Excellence for Clinical Research in Neurological Diseases, Haukeland University Hospital, 3Department of Neurosurgery, Qilu Hospital and Institute of Brain and Brain-Inspired Science, Cheeloo College of Medicine, Shandong University, 4Shandong Key Laboratory of Brain Function Remodeling
* These authors contributed equally

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This study reports a novel approach to measure multiple mitochondrial functional parameters based on flow cytometry and double staining with two fluorescent reporters or antibodies to detect changes in mitochondrial volume, mitochondrial membrane potential, reactive oxygen species level, mitochondrial respiratory chain composition, and mitochondrial DNA.

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Keywords: Flow Cytometry Mitochondrial Parameters Human Induced Pluripotent Stem Cells Neural Derivatives Glial Derivatives FCCP TMRE MTG Mito Sox Cell Dissociation Paraformaldehyde Methanol Blocking Buffer
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