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Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice

Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice

DOI:
10.3791/63125-v

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04:29 min

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July 01, 2022

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, , , , ,
1Tianjin Institute of Urology,The Second Hospital of Tianjin Medical University, 2Department of Pathology,The Second Hospital of Tianjin Medical University, 3Department of Urology,The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University

Capitoli

  • 00:17Introduction
  • 00:37Isolation of Bone Marrow and Preparation of BM Cells
  • 02:05Induction Culture of BMDC
  • 03:11Risultati
  • 03:59Conclusion

Summary

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Traduzione automatica

Here, we present an economical and efficient method to isolate and generate high-purity bone marrow-derived dendritic cells from mice after 7 days of culture with 10 ng/mL GM-CSF/IL-4.

Tags

Bone Marrow-derived Dendritic CellsIsolationCultureMouseBMDCCD11cCD80MSC2GM-CSFIL-4Cost-effectiveEfficient

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