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DetectSyn：一种快速、无偏倚的荧光方法，用于检测突触密度的变化
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Biochimica
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JoVE Journal Biochimica
DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density
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DetectSyn：一种快速、无偏倚的荧光方法，用于检测突触密度的变化

DOI:
10.3791/63139-v

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09:10 min

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July 22, 2022

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, ,
1Wake Forest Translational Alcohol Research Center,Wake Forest School of Medicine, 2Physiology and Pharmacology,Wake Forest School of Medicine

Capitoli

  • 00:04Introduction
  • 00:34Primary Antibody Incubation
  • 01:29Sample Washes, Secondary Antibody Incubation, Ligation, Amplification, and Mounting
  • 03:49Digital Imaging with a Confocal Microscope
  • 05:49Image Analysis
  • 07:41Results: Increased Synapse Formation by Rapid Antidepressants in the Presence of GABAB Agonists
  • 08:42Conclusion

Summary

Traduzione automatica

Traduzione automatica

DetectSyn是一种无偏倚的快速荧光测定，可测量治疗或疾病状态下相对突触（突触前和突触后接合）数量的变化。该技术利用了一种接近连接技术，该技术既可用于培养的神经元，也可用于固定组织。

Tags

DetectSynFluorescent MethodSynapse DensityProximity Ligation AssayPrimary AntibodySecondary AntibodyLigationAmplificationMounting

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