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监测成人 果蝇 肠道的肠道酸化
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Biologia
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JoVE Journal Biologia
Monitoring Gut Acidification in the Adult Drosophila Intestine
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监测成人 果蝇 肠道的肠道酸化

DOI:
10.3791/63141-v

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04:39 min

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October 11, 2021

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1Children’s Research Institute,University of Texas Southwestern Medical Center

Capitoli

  • 00:05Introduction
  • 00:58Preparing for the Gut Acidification Monitoring Assay
  • 01:54Gut Acidification Monitoring Assay
  • 02:30Mounting and Image Acquisition
  • 02:52Results: Gut Acidification Monitoring and Phylogeny of Gut Acidification Phenomenon
  • 03:56Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里，我们提出了一种标准化的方案，用于监测 黑腹果蝇 的肠道酸化，并具有最佳输出。我们首先将此方案用于 黑色果蝇 的肠道酸化监测，然后证明其在非模型 果蝇 物种中的使用。

Tags

Gut AcidificationDrosophila IntestineBromophenol BluePH SensingFood PreparationFly DissectionGut IsolationMicroscopyAcidification MonitoringAcidic GutNon-acidic GutPercentage Calculation

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