Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5

00:05Introduction
00:55Needle Loading
01:38Injections
07:53Results: Transduction of the Murine Neural Plate by Nano-Injection at Embryonic Day 7.5
10:07Conclusion

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In this protocol, we describe how to inject the mouse amniotic cavity at E7.5 with lentivirus, leading to uniform transduction of the entire neural plate, with minimal detrimental effects on survival or embryonic development.

Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5

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