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1Mahidol Vivax Research Unit, Faculty of Tropical Medicine,Mahidol University, 2Department of Molecular Tropical Medicine & Genetics, Faculty of Tropical Medicine,Mahidol University, 3Department of Internal Medicine, Morsani College of Medicine,University of South Florida, 4Mahidol Vivax Research Unit, Faculty of Tropical Medicine,Mahidol University
This protocol describes a sandwich enzyme-linked immunosorbent assay to detect salivary gland sporozoites in mosquitoes. Using easily available monoclonal antibodies, the method enables cost-effective, high-throughput detection of mosquitoes carrying Plasmodium falciparum or Plasmodium vivax. The method is suitable for malaria transmission research, including vector surveys.
