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前子宫 从前肠胚形成到晚期器官生成的小鼠胚胎培养
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Ex Utero Culture of Mouse Embryos from Pregastrulation to Advanced Organogenesis
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前子宫 从前肠胚形成到晚期器官生成的小鼠胚胎培养

DOI:
10.3791/63160-v

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07:14 min

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October 19, 2021

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,
1Department of Molecular Genetics,Weizmann Institute of Science

Capitoli

  • 00:04Introduction
  • 00:47Ex Utero Roller Culture of Embryos from Embryonic Day 7.5 to 11
  • 03:48Extended Embryo Culture from Embryonic Day 5.5/6.5 to 11
  • 04:38Results: Continuous and Robust Ex Utero Development of Post Implantation Mouse Embryos
  • 06:34Conclusion

Summary

Traduzione automatica

Traduzione automatica

增强的全胚胎培养平台允许植入后小鼠胚胎的连续和稳健的 宫外 发育长达六天，从前肠胚形成阶段到高级器官发生。在该协议中，我们详细介绍了使用静态板和旋转瓶系统成功培养胚胎的标准程序。

Tags

Keywords: Ex Utero CultureMouse EmbryosPregastrulationOrganogenesisGastrulationRoller Culture IncubatorGas ConcentrationGas PressureEmbryo DissectionCulture MediumDPBSPregnant Mouse

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