Rivista
/
Biologia
/
Investigating Cardiac Metabolism in the Isolated Perfused Mouse Heart with Hyperpolarized [1-13C]Pyruvate and 13C/31P NMR Spectroscopy
JoVE Journal
Biologia
Author Produced
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Investigating Cardiac Metabolism in the Isolated Perfused Mouse Heart with Hyperpolarized [1-13C]Pyruvate and 13C/31P NMR Spectroscopy

Investigating Cardiac Metabolism in the Isolated Perfused Mouse Heart with Hyperpolarized [1-13C]Pyruvate and 13C/31P NMR Spectroscopy

DOI:
10.3791/63188-v

•

14:56 min

•

April 21, 2023

•

, , , , ,
1Department of Radiology, Hadassah Medical Organization and Faculty of Medicine,Hebrew University of Jerusalem, 2The Wohl Institute for Translational Medicine

Capitoli

  • 00:03Introduction
  • 01:17Krebs-Henseleit Buffer Preparation
  • 01:45Perfusion System Preparation
  • 02:29Calibration and Preparation of the NMR Spectrometer for Acquisition
  • 03:22Animal Preparation, Surgical Procedure, and Perfusion of the Heart in the NMR Tube
  • 09:23Acquiring Data for Cardiac Energetics and pH
  • 10:09DNP Spin Polarization and Dissolution
  • 11:25Hyperpolarized 13C Spectroscopy
  • 12:07Representative Results
  • 14:40Conclusion

Summary

Traduzione automatica

Traduzione automatica

We describe an experimental setup for administrating hyperpolarized 13C-labeled metabolites in continuous perfusion mode to an isolated perfused mouse heart. A dedicated 13C-NMR acquisition approach enabled the quantification of metabolic enzyme activity in real-time, and a multiparametric 31P-NMR analysis enabled the determination of the tissue ATP content and pH.

Tags

Hyperpolarized MRICardiac MetabolismIsolated Perfused Mouse HeartPyruvate Metabolism13C/31P NMR SpectroscopyKrebs-Henseleit BufferAerobic And Anaerobic MetabolismCardiac EnergeticsPHLactateInorganic PhosphatePerfusion SystemTemperature Control

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/63188?article_type=v

Optical Mapping of Langendorff-perfused Rat Hearts
check_url/it/63188?article_type=v

Characterization of the Isolated, Ventilated, and Instrumented Mouse Lung Perfused with Pulsatile Flow
check_url/it/63188?article_type=v

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
check_url/it/63188?article_type=v

Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria
check_url/it/63188?article_type=v

Intravital Microscopy of the Microcirculation in the Mouse Cremaster Muscle for the Analysis of Peripheral Stem Cell Migration
check_url/it/63188?article_type=v

Confocal Imaging of Single Mitochondrial Superoxide Flashes in Intact Heart or In Vivo
check_url/it/63188?article_type=v

Quantifying Single Microvessel Permeability in Isolated Blood-perfused Rat Lung Preparation
check_url/it/63188?article_type=v

Optical Mapping of Intra-Sarcoplasmic Reticulum Ca2+ and Transmembrane Potential in the Langendorff-perfused Rabbit Heart
check_url/it/63188?article_type=v

The Mouse Isolated Perfused Kidney Technique
check_url/it/63188?article_type=v

Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart

Read Article