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Biochemistry

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JoVE Journal Biochemistry
Efficient and Scalable Production of Full-length Human Huntingtin Variants in Mammalian Cells using a Transient Expression System
 

Efficient and Scalable Production of Full-length Human Huntingtin Variants in Mammalian Cells using a Transient Expression System

Article DOI: 10.3791/63190-v • 10:52 min • December 10th, 2021
December 10th, 2021 •
1, 1, 1, 1, 1, 1, 1
1Discovery Biology, Curia Global Inc.

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We provide scalable protocols covering construct design, transient transfection, and expression and purification of full-length human huntingtin protein variants in HEK293 cells.

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Keywords: Huntingtin Transient Expression HEK293 Cells Polyethyleneimine Transfection Sodium Butyrate Anti-FLAG Purification FPLC Protein Production Huntington's Disease
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