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Using Alizarin Red Staining to Detect Chemically Induced Bone Loss in Zebrafish Larvae
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Using Alizarin Red Staining to Detect Chemically Induced Bone Loss in Zebrafish Larvae

Using Alizarin Red Staining to Detect Chemically Induced Bone Loss in Zebrafish Larvae

DOI:
10.3791/63251-v

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05:03 min

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December 28, 2021

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, , , , , , , ,
1Department of Toxicology, School of Public Health, Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases,Medical College of Soochow University, 2Department of Occupational Disease Prevention,Jiangsu Provincial Center for Disease Control and Prevention, 3Jiangsu Preventive Medicine Association, Nanjing, China

Capitoli

  • 00:04Introduction
  • 00:36Alizarin Red Staining
  • 02:05Image Acquisition
  • 02:38Image Analysis
  • 03:49Results: Bone Loss Detection Using Alizarin Red Staining
  • 04:36Conclusion

Summary

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Traduzione automatica

Here, we have used alizarin red staining to show that lead acetate exposure causes a bone mass change in zebrafish larvae. This staining method can be adapted to the investigation of bone loss in zebrafish larvae loss induced by other hazardous toxicants.

Tags

Keywords: Alizarin Red StainingZebrafish LarvaeBone ToxicityOsteoporosisSkeletal ChangesChemical ExposureImage AnalysisBone Mass Quantification

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