È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:04Introduction
- 00:56Preparation of Nude Mouse Ear Skin Tissue
- 01:46Preparation of Human Skin Tissue
- 02:26Lipid Imaging and Application of Topical Formulation
- 03:11Experimental Setup for Drug Quantification and Data Analysis
- 08:53Results: Analysis of the Skin for Tissue Movement and Concentration of Drug at Different Timepoints
- 10:28Conclusion
A coherent Raman scattering imaging methodology to visualize and quantify pharmaceutical compounds within the skin is described. This paper describes skin tissue preparation (human and mouse) and topical formulation application, image acquisition to quantify spatiotemporal concentration profiles, and preliminary pharmacokinetic analysis to assess topical drug delivery.
