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自发荧光成像评估细胞代谢
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JoVE Journal Bioingegneria
Autofluorescence Imaging to Evaluate Cellular Metabolism
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自发荧光成像评估细胞代谢

DOI:
10.3791/63282-v

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07:36 min

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November 15, 2021

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, , , ,
1Department of Biomedical Engineering,Texas A&M University-College Station

Capitoli

  • 00:04Introduction
  • 01:04Multiphoton Fluorescence Lifetime Imaging (FLIM) of Nicotinamide Adenine (Phosphate) Dinucleotide (NAD(P)H) and Flavin Adenine Dinucleotide (FAD)
  • 02:13Nicotinamide Adenine (Phosphate) Dinucleotide (NAD(P)H) Imaging
  • 03:35Flavin Adenine Dinucleotide (FAD) Imaging
  • 04:45Cyanide Experiment Preparation
  • 05:28Results: The Fluorescence Imaging of MCF-7 Cells Before and After Cyanide Treatment
  • 07:05Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议描述了内源性代谢辅酶，还原烟酰胺腺嘌呤（磷酸盐）二核苷酸（NAD（P）H）和氧化黄素腺嘌呤二核苷酸（FAD）的荧光成像和分析。NAD（P）H 和 FAD 的自发荧光成像提供了一种无标记、无损的方法来评估细胞代谢。

Tags

Keywords: AutofluorescenceNADHFADCellular MetabolismMulti-photon Fluorescence Lifetime MicroscopyLive Cell ImagingNon-destructive

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