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A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation
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1Department of Mechanical Engineering,Temple University, 2Department of Bioengineering,Temple University, 3Department of Bioengineering,University of Toledo, 4Biomedical Technology,CFD Research Corporation, 5Center for Inflammation and Lung Research, Department of Microbiology, Immunology and Inflammation, Lewis Katz School of Medicine,Temple University, 6Department of Radiation Oncology, Lewis Katz School of Medicine,Temple University
Capitoli
- 00:04Introduction
- 01:13Priming and Coating the Device with Human Fibronectin
- 03:01Seeding Biomimetic Microfluid Assay (bMFA) with HLMVEC
- 04:37Cell Culture Under Flow for 48 Hours
- 05:26Cytokine and/or Therapeutic Treatment
- 06:19Neutrophil Adhesion and Migration Experiment with bMFA
- 08:33Acquire Image
- 09:59Results: Biomimetic Microfluid Assay to Study Leukocyte-Endothelial Cell Interactions
- 12:06Conclusion
In this protocol, a biomimetic microfluid assay, which can reproduce a physiologically relevant microvascular environment and reproduce the entire leukocyte adhesion/migration cascade, is employed to study leukocyte-endothelial cell interactions in inflammatory disease.
Tags
Keywords:
Microphysiological SystemLeukocyte-endothelial Cell InteractionInflammationSepsisVascular Endothelium BarrierLeukocyte TraffickingOrgan DamageBiomimetic Microfluidic AssayPrimary Human CellsClinically Relevant Patient SamplesTherapeutic ScreeningNeutrophil RollingAdhesionSpreadingMigrationHuman FibronectinDegassingHuman Lung Microvascular Endothelial Cells
