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Hurtig indkapsling af rekonstitueret cytoskelet inde i gigantiske unilamellare vesikler
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1Department of Mechanical Engineering,University of Michigan, Ann Arbor, 2John A. Paulson School of Engineering and Applied Sciences,Harvard University, 3Department of Cellular and Molecular Biophysics,Max Planck Institute of Biochemistry, 4Department of Biomedical Engineering,University of Michigan, Ann Arbor, 5Department of Biophysics,University of Michigan, Ann Arbor, 6Cellular and Molecular Biology Program,University of Michigan, Ann Arbor
Capitoli
- 00:05Introduction
- 00:48Preparation of Oil-Lipid-Mixture
- 02:12Vesicle Generation
- 05:42Imaging and 3D image Reconstruction
- 06:53Results: Encapsulation of Actin Bundle Structures
- 07:08Conclusion
Denne artikel introducerer en simpel metode til hurtig produktion af gigantiske unilmellære vesikler med indkapslede cytoskeletale proteiner. Metoden viser sig at være nyttig til bottom-up rekonstitution af cytoskeletale strukturer i indespærring og cytoskelet-membraninteraktioner.
