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Encapsulación rápida del citoesqueleto reconstituido dentro de vesículas unilamelares gigantes
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1Department of Mechanical Engineering,University of Michigan, Ann Arbor, 2John A. Paulson School of Engineering and Applied Sciences,Harvard University, 3Department of Cellular and Molecular Biophysics,Max Planck Institute of Biochemistry, 4Department of Biomedical Engineering,University of Michigan, Ann Arbor, 5Department of Biophysics,University of Michigan, Ann Arbor, 6Cellular and Molecular Biology Program,University of Michigan, Ann Arbor
Capitoli
- 00:05Introduction
- 00:48Preparation of Oil-Lipid-Mixture
- 02:12Vesicle Generation
- 05:42Imaging and 3D image Reconstruction
- 06:53Results: Encapsulation of Actin Bundle Structures
- 07:08Conclusion
Este artículo presenta un método simple para la producción expedita de vesículas unilamelares gigantes con proteínas citoesqueléticas encapsuladas. El método demuestra ser útil para la reconstitución ascendente de estructuras citoesqueléticas en confinamiento e interacciones citoesqueleto-membrana.
