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Bioengineering

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JoVE Journal Bioengineering
Snabb inkapsling av rekonstituerad cytoskelett inuti jätte unilamellarblåsor
 
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Snabb inkapsling av rekonstituerad cytoskelett inuti jätte unilamellarblåsor

Article DOI: 10.3791/63332-v • 07:48 min • November 10th, 2021
November 10th, 2021 •
*1, *1, 2, 3, 1,4,5,6
1Department of Mechanical Engineering, University of Michigan, Ann Arbor, 2John A. Paulson School of Engineering and Applied Sciences, Harvard University, 3Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, 4Department of Biomedical Engineering, University of Michigan, Ann Arbor, 5Department of Biophysics, University of Michigan, Ann Arbor, 6Cellular and Molecular Biology Program, University of Michigan, Ann Arbor
* These authors contributed equally

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Denna artikel introducerar en enkel metod för snabb produktion av gigantiska unilamellarblåsor med inkapslade cytoskelettproteiner. Metoden visar sig vara användbar för bottom-up rekonstitution av cytoskelettstrukturer i inneslutning och cytoskelett-membraninteraktioner.

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Bioteknik utgåva 177 Emulsionsöverföring cDICE GUVs bottom-up rekonstituering aktin fascin
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