Rivista
/
Biologia
/
从小鼠骨骼肌中分离线粒体用于呼吸测定
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Isolation of Mitochondria from Mouse Skeletal Muscle for Respirometric Assays
Please note that all translations are automatically generated. Click here for the English version.

从小鼠骨骼肌中分离线粒体用于呼吸测定

DOI:
10.3791/63336-v

•

08:11 min

•

February 10, 2022

•

, , , , ,
1Centro Andaluz de Biología del Desarrollo, CSIC-UPO-JA,Universidad Pablo de Olavide, 2CIBERER,Instituto de Salud Carlos III

Capitoli

  • 00:05Introduction
  • 00:53Muscle Dissection, Homogenization, and Mitochondrial Isolation
  • 04:35Preparation of the Microplate-Based Respirometric Assays
  • 06:17Results: Mitochondria Isolation from Mouse Skeletal Muscle
  • 07:32Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里，我们描述了从小鼠骨骼肌中分离线粒体的详细方法，以及随后使用基于微孔板的呼吸测定通过耗氧率（OCR）分析呼吸。该管道可用于研究多种环境或遗传干预措施对线粒体代谢的影响。

Tags

Mitochondria IsolationRespirometric AssaysSkeletal MuscleMousePurificationRespiration AnalysisExperimental ConditionsOrganismAgingDrug TestingCancerDevelopmentPBSEDTAIB1DissectionSkeletal MusclesTibialis AnteriorExtensor Digitorum LongusGastrocnemiusSoleus

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/63336?article_type=v

成人和胚胎骨骼的肌肉Microexplant文化和骨骼肌肉干细胞的分离
check_url/it/63336?article_type=v

骨骼肌的线粒体分离
check_url/it/63336?article_type=v

纯化的骨骼肌的先觉者
check_url/it/63336?article_type=v

激光伤害的斑马鱼胚胎骨骼肌
check_url/it/63336?article_type=v

单个肌纤维和成人骨骼肌卫星细胞的分离和培养
check_url/it/63336?article_type=v

Analysis of Skeletal Muscle Defects in Larval Zebrafish by Birefringence and Touch-evoke Escape Response Assays
check_url/it/63336?article_type=v

从人骨骼肌隔离血管源性多能前体
check_url/it/63336?article_type=v

隔离和线粒体从培养细胞和小鼠组织功能分析
check_url/it/63336?article_type=v

从小鼠骨骼肌高通量微孔板呼吸测量最低数量的隔离线粒体
check_url/it/63336?article_type=v

骨骼肌完整的线粒体的分离差速离心高分辨率测量呼吸运动

Read Article