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记录来自 非洲爪蟾 卵母细胞的间隙结电流
JoVE Journal
Biologia
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JoVE Journal Biologia
Recording Gap Junction Current from Xenopus Oocytes
DOI:

09:04 min

January 21, 2022

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Capitoli

  • 00:04Introduction
  • 00:42Preparation of Defolliculated Oocytes
  • 01:27GJ Protein Expression
  • 03:03Testing the Acquisition System with the Model Cell Supplied with the Amplifier
  • 04:09Set Up the Recording Stage and the Reference Electrode
  • 04:48Set Up the VDIFF Electrodes
  • 05:51Set Up the Current, Voltage Electrodes, Data Acquisition
  • 06:57Results: Representative Recording Traces
  • 08:05Conclusion

Summary

Traduzione automatica

在这里,我们提出了一种方案,用于表达 非洲爪蟾 卵母细胞中的间隙连接蛋白,并使用商业放大器记录两个附着的卵母细胞之间的连接电流,该放大器设计用于在高压侧电流测量模式下记录双卵母细胞电压钳位记录。

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