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Studying Membrane Protein Trafficking in Drosophila Photoreceptor Cells Using eGFP-Tagged Proteins
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Biologia
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JoVE Journal Biologia
Studying Membrane Protein Trafficking in Drosophila Photoreceptor Cells Using eGFP-Tagged Proteins

Studying Membrane Protein Trafficking in Drosophila Photoreceptor Cells Using eGFP-Tagged Proteins

DOI:
10.3791/63375-v

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10:20 min

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January 21, 2022

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, , ,
1Institute of Biology, Department of Biochemistry,University of Hohenheim

Capitoli

  • 00:04Introduction
  • 01:26Deep Pseudopupil (DPP) Imaging
  • 02:34Water Immersion Microscopy
  • 07:47Results: Genetic Screens for Drosophila Mutants Defective in TRPL Translocation and Assessment of Retinal Degeneration in Drosophila Photoreceptor Cells
  • 09:26Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, non-invasive methods are described for localization of photoreceptor membrane proteins and assessment of retinal degeneration in the Drosophila compound eye using eGFP fluorescence.

Tags

Membrane Protein TraffickingDrosophila Photoreceptor CellsEGFP-tagged ProteinsTRPL GFPPhotoreceptor DegenerationDeep Pseudopupil (DPP) ImagingWater-immersion MicroscopyOmmatidiaFluorescence ImagingAnesthetization

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