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Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy
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Biochimica
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JoVE Journal Biochimica
Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy

Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy

DOI:
10.3791/63377-v

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07:20 min

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February 18, 2022

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, ,
1Biologia Molecolare,Massachusetts General Hospital, 2Department of Genetics,Harvard Medical School

Capitoli

  • 00:04Introduction
  • 01:05Microtubule Visualization via TIRF Microscopy
  • 01:58Microscope Settings
  • 03:17Generation of Surface-Immobilized Microtubule Bundles
  • 04:57Image Microtubule Dynamics
  • 05:53Results: TIRF Microscopy of Crosslinked and Single Microtubules
  • 06:35Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, a TIRF microscopy-based in vitro reconstitution assay is presented to simultaneously quantify and compare the dynamics of two microtubule populations. A method is described to simultaneously view the collective activity of multiple microtubule-associated proteins on crosslinked microtubule bundles and single microtubules.

Tags

MicrotubulesTIRF MicroscopyMulti-component ReconstitutionMicrotubule DynamicsMicrotubule SubpopulationsSingle MicrotubulesMicrotubule BundlesFluorescent LabelingExperimental ParametersImaging Setup

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