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测量成年小鼠急性纹状体切片中的耗氧率
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Measurement of Oxygen Consumption Rate in Acute Striatal Slices from Adult Mice
DOI:

07:41 min

June 08, 2022

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Capitoli

  • 00:04Introduction
  • 00:57Hydrating the Cartridge Sensors
  • 01:45Preparing the Tissue Plate
  • 02:24Acute Striatal Slice Preparation and Excised Slice Placement
  • 04:11Loading of Cartridges with Desired Compounds
  • 05:17Calibration and Performing the Assay
  • 06:07Results: Measuring the Oxygen Consumption Rates in Acute Striatal Slices from Adult Mice
  • 07:11Conclusion

Summary

Traduzione automatica

耗氧率(OCR)是线粒体功能的常见代理,可用于研究不同的疾病模型。我们开发了一种使用Seahorse XF分析仪的新方法,直接测量成年小鼠急性纹状体切片中的OCR,该方法比其他方法更具生理相关性。

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