È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:00Introduction
- 00:24Selection of the Most Potent Promoter to Obtain Persistent and Prolonged Expression of the shRNAs
- 02:01Preparation of Pooled Lentiviral Human Epigenetic Factor shRNA Library
- 04:48Estimation of the Transduction Efficiency of Lentiviruses
- 06:03Transduction of Pooled Epigenetic shRNA Library in the Drug-Resistant Cell Line
- 07:20Enrichment of GFP Positive Cells
- 07:54Dropout Screening to Identify Epigenetic Factors Mediating Drug Resistance
- 09:20Amplification of the Integrated shRNAs by PCR
- 11:08Next-Generation Sequencing and Data Analysis
- 11:57Representative Results
- 14:30Conclusion
