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快速高尔基染色用于海马体和前额叶皮层树突状脊柱可视化

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Rapid Golgi Stain for Dendritic Spine Visualization in Hippocampus and Prefrontal Cortex

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快速高尔基染色用于海马体和前额叶皮层树突状脊柱可视化

DOI:

10.3791/63404-v

•

04:58 min

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December 03, 2021

•

Maya Frankfurt², Rachel Bowman

¹Department of Science Education,Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, ²Department of Psychology,Sacred Heart University

Capitoli

00:04Introduction
00:48Sectioning of Brain Tissues and Mounting
02:04Staining and Dehydration of Brain Tissue
03:06Determination of Dendritic Spine Density
03:48Results: Observation of Hippocampus and Effect of Environmental Enrichment on CA1 and mPFC
04:23Conclusion

Summary

Traduzione automatica

该协议描述了快速高尔基体方法的修改，该方法可以适应神经系统的任何部分，用于染色大鼠海马体和内侧前额叶皮层中的神经元。

Tags

Golgi Stain Dendritic Spine Hippocampus Prefrontal Cortex Cryostat Sectioning Rapid Labeling Golgi Impregnation Neuron Analysis Dendritic Length Spine Density

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