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In vivo Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy
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1Department of Electronic and Computer Engineering,The Hong Kong University of Science and Technology, 2State Key Laboratory of Molecular Neuroscience,The Hong Kong University of Science and Technology, 3Center of Systems Biology and Human Health,The Hong Kong University of Science and Technology, 4Molecular Neuroscience Center,The Hong Kong University of Science and Technology, 5Department of Biology, School of Life Sciences,Southern University of Science and Technology
Capitoli
- 00:04Introduction
- 00:59Starting-Up the Lasers
- 02:02Optical Alignment of the Combined TPEF-Stimulated Raman Scattering Microscope
- 04:39Optimizing Imaging Conditions
- 06:36In Vivo TPEF-SRS Imaging of Mouse Spinal Cord
- 08:03Results: In Vivo Dual-Modal Imaging of Spinal Axons and Myelin Sheaths
- 08:31Conclusion
Stimulated Raman scattering (SRS) microscopy allows label-free imaging of biomolecules based on their intrinsic vibration of specific chemical bonds. In this protocol, the instrumental setup of an integrated SRS and two-photon fluorescence microscope is described to visualize cellular structures in the spinal cord of live mice.
