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一种用于乳腺肿瘤微环境活体内成像的无标记分割方法

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Ricerca sul cancro

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JoVE Journal Ricerca sul cancro

A Label-Free Segmentation Approach for Intravital Imaging of Mammary Tumor Microenvironment

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一种用于乳腺肿瘤微环境活体内成像的无标记分割方法

DOI:

10.3791/63413-v

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10:39 min

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May 24, 2022

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Brian M. Burkel, David R. Inman, María Virumbrales-Muñoz², Erica J. Hoffmann, Suzanne M. Ponik³

¹Department of Cell and Regenerative Biology,University of Wisconsin-Madison, ²Department of Pathology,University of Wisconsin-Madison, ³Carbone Cancer Center,University of Wisconsin-Madison

Capitoli

00:04introduction
00:55Generation and Surgical Implantation of the Mammary Imaging Window (MIW)
04:13Positioning and Maintaining Mouse on the Microscope Stage for Imaging
05:52Set Up for 4D, Intensity-Based, Label-Free Intravital Imaging of Dynamic Cell Behavior
08:02Fluorescence Lifetime Imaging (FLIM) of NAD(P)H
08:43Results: Intravital Imaging of the Mammary Tumor Microenvironment
10:15Conclusion

Summary

Traduzione automatica

本文描述的活体成像方法利用来自代谢辅助因子NAD（P）H的胶原二次谐波和内源性荧光，将未标记的肿瘤微环境非侵入性地分割成肿瘤，基质和血管室，以深入分析4D活体内图像。

Tags

Keywords: Intravital Imaging Mammary Tumor Microenvironment Label-free Segmentation Collagen Fibers Autofluorescent Metabolites Extracellular Matrix Vascular Structures Mammary Imaging Window Surgical Procedure Mouse

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